ORIGINAL RESEARCH
Utilization of Egg Shell Waste in Cellulase
Production by Neurospora crassa under Wheat
Bran-Based Solid State Fermentation
Nitin Verma, Vivek Kumar, Mukesh C. Bansal
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Department of Paper Technology, Indian Institute of Technology,
Roorkee Saharanpur Campus, Saharanpur, U.P, 247001, India
Pol. J. Environ. Stud. 2012;21(2):491-497
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ABSTRACT
A wide variety of waste resources are available on our planet. Egg shell waste is available in huge quantities
from the food processing, egg breaking, and the hatching industries. Calcium plays a vital role in
Neurospora crassa hyphal growth. Batch experiments have been performed using pure calcium salt as well as
calcium in the form of pretreated egg shell waste in concentrations of 0.4, 0.8, and 1.2 g/l, under wheat branbased
solid state fermentation by Neurospora crassa at 30ºC with initial pH 6.0. The present paper describes
the utility of wheat bran and egg shell waste in cellulase production. The utilization of economically cheap and
abundantly available egg shell waste for cellulase production could be a novel and valuable approach in solid
waste management.