Aluminum (Al) is a widely found metal with no known biological or clinical benefits, and thus causes considerable toxicological effects on biological organisms’ health, including yeast. Mitochondria are the main cellular organelles involved in the production of oxygen and reactive oxygen species (ROS) in the cell. Yeast is the most scientifically used micro-ecologic, and the most sensitive microorganisms to various toxicants. As the toxicological effects of Al and mitochondrial respiratory chain inhibitors on Saccharomyces cerevisiae have not been elucidated; the present study was therefore, devoted to comparing the toxic effects of Al salts and three mitochondrial inhibitors, namely trimetazidine, Prednisolone, and Potassium Cyanide on Saccharomyces cerevisiae. Cells were exposed 2h increasing concentrations of Al (C1 = 0.017g/L, C2 = 0.034g/L, C3 = 0.342 g/L, and C4 = 8,5 g/L), and one concentration of mitochondrial inhibitor C1 = 0.0032g/L Potassium Cyanide (KCN), C1 = 0.013 g/L Trimetazidine (TMZ), and C1 = 0.018 g/L Prednisolone (PDN). Results showed an inhibition of cell growth in Al2 (SO4)3 and the used mitochondrial inhibitors, in particular, Cyanide and Prednisolone as evidenced by positive response percentages in exposed yeasts. Moreover, oxidative stress induction in Aluminum sulfate treatment was revealed by stimulation of catalase (CAT) and Glutathione S-transferase (GST) activity, along with increased levels of GSH and MDA compared with control.