Mycological cleanliness of rooms directly translates into the health of their users. This is important for athletes who are actively training. The aim of the study was to evaluate the sanitary and epidemiological status of sports changing rooms by determining the species diversity of their mycobiota in the context of prevention of athletes' health. The research was carried out in changing rooms of sports halls used by volleyball players in the city of Olsztyn (NE Poland). Standard mycological isolation and identification methods were used in this study. A total of 87 strains of microfungi were isolated. 52 belonged to the mold fungi, among which 18 species, belonging to 10 were found. The species isolated most frequently were: Aspergillus fumigatus, A. niger, Juxtiphoma eupyrena (previous name: Phoma eupyrena) and Westerdykella minutispora (previous name: Ph. minutispora). 35 yeast strains, belonging to 15 species and 10 genera were isolated. The most common species were: Candida glabrata, C. krusei, C. parapsilosis and Rhodotorula glutinis. 5 species were categorized as class BSL-2 fungi. The conducted research justify the need for preventive examinations of athletes and shows the necessity of constant monitoring of the sanitary condition of the sport changing rooms.