ORIGINAL RESEARCH
The Mitigation Effects of Exogenous Hydrogen
Peroxide when Alleviating Seed Germination
and Seedling Growth Inhibition
on Salinity-Induced Stress in Barley
Semra Kilic, Ayten Kahraman
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Department of Biology, Faculty of Arts and Science, Süleyman Demirel University,
32260, Isparta, Turkey
Submission date: 2015-12-09
Final revision date: 2016-02-18
Acceptance date: 2016-02-18
Publication date: 2016-05-25
Pol. J. Environ. Stud. 2016;25(3):1053-1059
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ABSTRACT
Our study focused on the mitigation role of exogenously applied hydrogen peroxide (H2O2) in eliminating
toxicty caused by salt (NaCl). Barley seeds were pretreated with 30 μM (micromolal) H2O2 for 24 hours
and then exposed to increasing salt concentrations (0.0, 0.25, 0.275, 0.30 M). Morphogical and physiological
changes in seed germination and seedling growth stages were compared between different treatments
of salt in laboratory conditions. Adverse effects of salt during both germination and seedling growth stages
were dependent on the concentration of the salt treatment. We found that the application of H2O2 effectively
alleviated the salt-induced inhibition, and reduced the negative effects of salt on germination (germination
index and vigor index), seedling growth stages (radicle and coleoptile lengths, fresh weight), and leaf
parameters (stomata and epidermis counts, stomatal index, stomata sizes of adaxial and abaxial surfaces).
The differences were statistically signifi cant. Alleviating the effects of H2O2 increased in parallel with salt
concentration (p≤0.05). However, under non-stress conditions (control), H2O2 didn’t have any effect on the
investigated parameters (p≥0.05). Our results suggest that exogenous H2O2 application is involved in the
resistance of barley to salt stress.