ORIGINAL RESEARCH
The Isolation and Characterization
of Glutamicibacter DC1 to Induce Carbonate
Precipitation of Some Heavy Metals
at Low-Temperature
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1
College of Resource and Environment, Jilin Agricultural University, Changchun 130118, China
2
College of Horticulture, Jilin Agricultural University, Changchun 130118, China
Submission date: 2021-07-06
Final revision date: 2021-09-19
Acceptance date: 2021-09-27
Online publication date: 2022-02-04
Publication date: 2022-03-22
Corresponding author
Chunli Zhao
College of Horticulture, Jilin Agricultural University, 130118, China
Pol. J. Environ. Stud. 2022;31(2):1693-1703
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ABSTRACT
The psychrotolerant bacteria able to induce carbonate precipitation of heavy metals at low
temperatures play an essential role in the remediation of heavy metal contaminated soil in cold regions.
In this study, a psychrotolerant bacterial strain DC1 with carbonate mineralization potential, identified
as Glutamicibacter sp., was isolated from tailing soil. The optimal temperatures and pH range for the
growth of strain DC1 were 10-30ºC and 6-8, respectively. Besides, strain DC1 showed a high degree
of resistance to various metals (Pb>Cu>Ni>Cd). Furthermore, strain DC1 produced a large amount of
urease (266.91 U/mL), which induced the fermentation suspension’s pH (pH 9.5 at 10ºC). Moreover,
the fermentation suspension of strain DC1 could generate carbonate precipitates of various metals (Pb,
Cd, Ni, and Cu) to remove them from the solutions. Based on the analyses of XRD, SEM, and EDS,
the carbonate precipitates of Pb and Cd have been identified as PbCO3 and CdCO3 mineral crystals,
respectively. In contrast, no mineral crystals of Ni and Cu existed. Furthermore, the carbonate minerals
of Cd and Pb with regular crystal improved resistance to dilute acid. Thus, this study showed that
strain DC1 without acid production could induce carbonate precipitates of Pb, Cd, Cu, and Ni at lowtemperature.