The content of 38 microelements and trace elements in raw milk of cows from Lower Silesia (LS) and Upper Silesia (US) were examined. Analytical method of mass spectrometry (ICP-MS) with microwave sample digestion was used in this study. Statistically significant differences were detected in the case of iodine, rubidium, cesium, tungsten and thorium (more in the US region) as well as aluminum, titanium, manganese, gallium, selenium, germanium, cobalt (more in LS). No statistically significant differences were observed in the case of: Zn, Ba, Cu, Cr, V, Ni, As, Mo, Pt, Sb, Au, Hf, Ce, U, Re, Tl, Ru, Rh, Ir, Ta and Be. For La, Nb, Ag, In and Y statistical differences were not calculated since in the majority of samples concentrations were below detection limit.