Tissue culture techniques in tomato, pepper and eggplant are important for the development of disease-resistant and high yielding varieties, which require a suitable regeneration protocol. Although shoot regeneration has been achieved by using different explants and cytokinin doses in Solanaceae species, very few studies have reported in vitro regeneration using root tissues. The current study is the first report to compare direct shoot regeneration capabilities using root node explants in three Solanaceae species (tomato, pepper, and eggplant) under three cytokinins (BAP, TDZ, and GA3) hormone. Plantlets were regenerated from the root node explants of tomato, pepper and eggplant in the media containing 0, 0.5, 1, 1.5 and 2mg/L of BAP, TDZ and GA3. Results revealed that the shoot regeneration of root node explants varied according to the species, cytokinins (BAP, TDZ and GA3) and doses of hormones. Among the species, the best shoot regeneration was observed in tomato followed by eggplant and pepper plants. While the shoot length was statistically significant in tomato, it was observed to be insignificant in pepper and eggplant. The highest number of root regeneration and root length was observed in tomatoes. The results obtained from the study will contribute to the development of successful/reproducible tissue culture protocols from roots node explants.