ORIGINAL RESEARCH
Spatial Variability of Total and Mineral Nitrogen
Content and Activities of the N-Cycle Enzymes
in a Luvisol Topsoil
Anna Piotrowska
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Department of Biochemistry, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences,
Bernardyńska, 6, 85-029 Bydgoszcz, Poland
Pol. J. Environ. Stud. 2011;20(6):1565-1573
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ABSTRACT
The aim of this study was to evaluate and compare the spatial variability of total nitrogen (TN), mineral
nitrogen content (N-NO3 and N-NH4) and activities of the enzymes involved in the soil N cycle (urease –
UR, nitrate reductase – NR, arginine deaminase activity – ADA) in a Luvisol surface horizon. Fifty soil samples
were collected in a square sampling grid (10 m x 10 m) system. The results were evaluated using classic
statistical and geostatistical methods. Most of the studied properties were distributed normally with the exception
of NR activity and nitrate nitrogen (N-NO3) content, which were distributed log-normally. Variation of
coefficients (CV%) for total and mineral nitrogen forms was low, for UR and ADA activities moderate, and
for NR activity high. The contribution of nugget variance in total variability (sill) was noted for UR and NR
activities, and for TN content. The ranges of spatial influence calculated for the values of the studied properties
ranged from 10-40 m. Kriged maps showed that soil UR and ADA activities and N-NO3 content were
irregularly distributed in the soil surface horizon, while more regular spatial distribution was noted for NR
activity and for TN and ammonium nitrate (N-NH4) content.