ORIGINAL RESEARCH
Soil Microbial Functional Diversity Responses
to Different Revegetation Types
in Baishilazi Nature Reserve
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1
College of Forestry, Shenyang Agriculture University, Shenyang, China
2
College of Land and Environment, Shenyang Agriculture University, Shenyang, China
3
Research Station of Liaohe-River Plain Forest Ecosystem, Chinese Forest Ecosystem Research Network (CFERN),
Shenyang Agricultural University, Changtu, China
4
Liaoning Baishi Lazi National Nature Reserve Administration, Dandong, China
Submission date: 2018-09-01
Final revision date: 2018-10-10
Acceptance date: 2018-10-21
Online publication date: 2019-05-14
Publication date: 2019-07-08
Corresponding author
Wenxu Zhu
College of Foresty, Shenyang Agriculture University, No.120,Dongling Road, Shenhe District, Shenyang City, Liaoning Province,China, zhuwenxu.315@163.com Shenyang, China
Pol. J. Environ. Stud. 2019;28(5):3675-3686
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ABSTRACT
Soil microorganisms play important roles in the dynamic regulation of organic matter in
the forest ecosystem and are affected by different revegetation types. To reveal the influence
of different revegetation types on soil microorganisms, we examined soil properties, soil microbial
activity and diversity in Baishilazi Nature Reserve, including two planted coniferous forests (LG: Larix
gmelinii, PK: Pinus koraiensis), two natural secondary broadleaf forests (JM: Juglans mandshurica,
QM: Quercus mongolica), and one conifer-broadleaf forest (CB). Biolog-Eco plates were used to
study soil microbial functional diversity. We found that the content of soil total C and total N existed
higher under the broadleaf forests (JM, QM) than conifer-broadleaf forest (CB) and coniferous
forests (LG, PK). Carbon source utilization capacity and soil microbial activity showed significant
variations among different revegetation types. Soil microbial activity of natural secondary forests
was significantly higher than planted coniferous forests, and JM created the highest soil microbial
activity. Heatmap and PCA plot clearly differentiated among the different samples. The broadleaf
forests, conifer-broadleaf forest and coniferous forests were well separated from each other,
especially along the PC1, and the position of conifer-broadleaf forest was intermediate. The findings
of canonical correspondence analysis (CCA) suggested that soil total C and total N were the main
factors affecting soil microbial functional diversity. This study investigated how shifts in soil microbial
functional diversity affected by different revegetation types were operational indicators of soil quality
in Baishilazi Nature Reserve and that the JM created the highest carbon source utilization soil microbial
diversity.