ORIGINAL RESEARCH
Soil Microbe and Physicochemical Characteristics
in Tensile Fracture Zone Caused
by Mining Subsidence
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School of Earth and Environment, Anhui University of Science and Technology,
168 Taifeng Street, Huainan, China, 232001
Submission date: 2022-09-14
Final revision date: 2022-11-18
Acceptance date: 2022-12-28
Online publication date: 2023-02-14
Publication date: 2023-04-14
Corresponding author
Tingyu Fan
Anhui University of Science and Technology, China
Pol. J. Environ. Stud. 2023;32(3):2361-2372
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ABSTRACT
The bacterial community plays a crucial role in the soil ecosystem, and is closely linked with soil
physicochemical properties. Surface tension cracks caused by underground coal mining inevitably
have a significant impact on physical properties. To reveal the characteristics of the soil bacterial
communities in the surface tension zones, high-throughput 16S rRNA sequencing was used to analyze
the bacterial communities in surface tension fracture areas. Furthermore, the relationships between
environmental factors and the diversity and abundance of the bacterial community, alpha diversity
index, and variations in bacterial communities were discussed. We analyzed soil bacterial community
structure and soil physicochemical properties from different dimensions. The results indicated that
The abundance of bacteria in the soil surface layer was higher, and the nutrients in the middle and
bottom of the slope increased with the increase of soil depth. Additionally, our data illustrated that
microorganisms participated in soil carbon and nitrogen cycling in the soil samples. This study provides
a reference for the study of variations in the bacterial diversity and community composition in surface
tension fracture areas caused by underground mining.