Our study evaluates the susceptibility of 17 strains of mould isolated from the soil of allotments in Łódź, Poland to 6 types of herbicide: Roundup Ultra 170 SL, Mniszek 540 SL, Casoron 6.75 GR, Basta 150 SL, Fusilade Forte 150 EC, and Starane 250 EC. Of these herbicides, only Casoron 6.75 GR is used in agriculture on the root system, whereas all the others are used on the leaf surface. Isolated strains of fungi belonged to 9 species: Fusarium aquaeductuum, Fusarium oxysporum, Mucor indicus, Penicillium chrysogenum, Penicillium citrinum, Penicillium purpurogeum, Penicillium rugulosum, Penicillium spinulosum, and Scedosporium prolificans. The herbicide Basta 150 SL is the strongest inhibitor of the growth of the examined fungi strains, as well as being the fastest herbicidal agent (2-5 days after use), and can be used to eradicate mould fungi in soil.