The aim of this study was to determine the species composition and concentrations of mites in dust collected from sleeping places in urban and rural apartments of northwestern Poland. In the dust samples from both urban and rural apartments the following mites were determined: Dermatophagoides farinae Hughes, D. pteronyssinus (Trouessart), Euroglyphus maynei (Cooreman), Hirstia sp., Chortoglyphus arcuatus (Troupeau), Lepidoglyphus destructor (Schrank), Gohieria fusca (Oudemans), and Cheyletus sp. The obtained results confirm high concentrations of mites in sleeping places both in urban and rural apartments, and the influence of environmental conditions and the age and building type on the composition of acarofauna.