Freshwater ecosystems have long been affected by numerous types of human interventions that have a negative impact. Ecosystem disturbance constitutes the worst aquatic response to anthropogenic pressures. The research was initiated by identifying the human pressures on the lakes’ ecosystem, the main objective being to establish, quantify and diminish the aquatic ecosystem responses. The case study was applied in the suburban area of Craiova city for a natural protected area. The chosen methodology is based on the analysis of the response of the 10 ten lakes located along Preajba Valley to human pressures from two perspectives: morphometric parameters of the lakes and indicators of the aquatic condition. For this purpose, a statistical analysis using Google Earth and GIS tools was conducted. The research was completed by field observations during 2009-2019. The results of the study draw attention to the lacustrine surface, its size decreasing from 28 ha in 2009 to 27 ha in 2019. The aquatic vegetation response was completed by determining the tendency of the vegetation extension (which has been ascending for Lakes II, III, VI and X). Following the interpretation of quantitative biocenotic indices, it can be concluded that the species Carassius gibelio is constant (Frequency-86.8% and Abundence-51.8%), being closely followed by Perca fluviatilis and Lepomis gibbosus as accessory species. Our results support the evidence that the high pH (8.2 pH unit), fixed residue (384 mg/dm³), NO² (0.124 mg/dm³), NO³ (16.28 mg/dm³) and CCOCr (49.7 mg/dm³) confirms the high degree of eutrophication in 2019. The paper suggested the assessment of lakes responses to human pressures as a support tool for sustainable lake ecosystems management in suburban areas.