Oil-degrading bacteria were found in soil samples polluted with oil in the oil tanker accident location and identified using biochemical tests and morphological analysis; fourteen bacterial isolates were identified. Bacterial isolates were identified at the molecular level using a universal primer, 16S rDNA gene. For soil samples, gas chromatography was used to evaluate total petroleum hydrocarbons. Heavy metal contamination (Cu, Cd, Mn, Zn, and Pb) was examined in all soil samples. CFU/g of the bacterial growth count ranged from 2.49×105 to 1.87×1017. The following bacterial genera were identified: Staphylococcus, Pseudomonas, Rhodococcus, Serratia, Bacillus, Acetobacter, Micrococcus, and Lactobacillus. Lactobacillus casei, Micrococcus luteus, and Pseudomonas fluorescens showed high growth rates on different types of hydrocarbons, such as toluene, naphthalene, and hexane. The highest Fe, Cd, Pb, Mn, and Cu concentrations were found in soil sample M2B. However, M1A has the highest Zn, Cu, and Fe concentrations. However, M1C has the lowest heavy metal concentrations. M2B, however, has the lowest Zn content. Pseudomonas species, which exhibit the greatest oil-degrading ability among all the isolates, may be utilized for bioremediation, as confirmed by this study. At one accident site, a high concentration of Cu showed high toxicity and low ability for hydrocarbon degradation in all bacterial isolates. Oil-contaminated locations might be cleaned up both in-situ and ex-situ using the species found in this study.