ORIGINAL RESEARCH
Investigation of the Valorization of Methanolic
Extract of Punica granatum L. Peel in Terms
of Phytochemical, Trace Element, Antioxidant
Activities and ADMET Profile
of Active Compounds
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1
Department of Biology, College of Science, University of Ha’il, P.O. Box 2440, Ha’il 2440, Saudi Arabia
2
Laboratory of Bioactive Substances, Center of Biotechnology of Borj Cedria, BP-901, 2050 Hammam-lif, Tunisia
3
University of Tunis-El Manar, Campus Universitaire Farhat Hached, BP-94 Rommana, 1068 Tunis, Tunisia
4
Center of Excellence in Desalination Technology, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia
5
Department of Computer Engineering, College of Computer Science and Engineering, University of Ha’il, KSA
6
Innov’COM, SupCom, Carthage University, Tunis, Tunisia
7
Section of Histology - Cytology, Medicine College of Tunis, Tunis El Manar University, Road Djebel Lakhdhar,
1007 La Rabta-Tunis, Tunisia
These authors had equal contribution to this work
Submission date: 2023-06-17
Final revision date: 2023-08-09
Acceptance date: 2023-11-08
Online publication date: 2024-02-09
Publication date: 2024-03-18
Corresponding author
Salem Elkahoui
Department of Biology, College of Science, University of Ha’il, P.O. Box 2440, Ha’il 2440, Saudi Arabia
Pol. J. Environ. Stud. 2024;33(3):2587-2602
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ABSTRACT
In this study, to valorize the pomegranate peels, the phytochemical composition of the methanolic
extract of Punica granatum L. peel (PPME) was characterized by high-resolution liquid chromatographymass
spectrometry (LC/HRMS), while the mineral composition of the extract was determined by ICP
AES. In the methanolic pomegranate peel extract, thirty-five different compounds were identified
by LC/HRMS, and it was determined that these compounds were mainly terpenoids, lipids and flavonoids.
Total polyphenol, flavonoid, and tannins contents of the PPME extract were determined as 211.43 mg
GAE/g polyphenol, 20.7 mg QE/g flavonoids and 11.5 mg TEA/g tannins. In order to determine the
antioxidant capacity of the extract, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH.) and
reducing power activity assays. These colorimetric assays showed that PPME has strong reducing power
and anti-radical capacity against DPPH with the IC50 values 0.210±0.005 mg/mL and 0.3±0.0045 mg/mL, respectively. Our findings clearly show that the methanolic extract of PPME contains two potent
compounds against gastric ulcers compared to the reference drug, esomeprazole.