This article provides information on moisture and impurities in cotton that is picked by hand and by different cotton-picking machines, as well as the number of knots in the hand and machine-made cotton fiber, in addition to the length of the staple of the fiber. An analysis of the results of manual and machine research on cotton is presented. The moisture content, the contamination, the number of knots, and the length of the staple depend on a number of factors, and the results obtained by the methods of their determination are illustrated. The quality of cotton is determined not only by the types of machines, but also by the climatic conditions during the harvest, cotton opening, field preparation, and other factors, as well as the contamination by the State Standard UzDst 592-2008 on the methods of determining the cotton contamination. The results of a practical study of comparing moisture and contamination of cotton harvested by hand and by cotton picking machine ‘’Case-2020’’ in Genefon breeder type 1 in the Dustlik district of Jizzakh region of Uzbekistan and contrasting type cotton harvested by John Deere by SPD90 and humidity and humidity indicators of hand-picked cotton and number of blanks and length of staples in cotton fiber JCS Jizzakh textile The analysis of the results of the tests performed on the laboratory equipment on the USTER AFIS PRO 2 equipment installed in the laboratory of the division is presented in table and graphical form. The range of contamination of cotton during its collection by various methods was established, which amounted to 7.1-10.0%. Based on the results of the research, it is stated that it is necessary to develop recommendations on the technological processes of cotton processing and spinning based on the analysis of moisture content, contamination, number of sticks, and staple lengths of cotton.