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eISSN: 2083-5906
ISSN: 1230-1485
ISSN: 1230-1485
ORIGINAL RESEARCH
Individual and Combined Effects of Sublethal
Cadmium and Lead on Tissue Accumulation,
Hemato-Biochemical Parameters and
Neurotoxicity in Channa marulius
1
Department of Zoology, Shah Abdul Latif University, Khairpur, 66020, Khairpur, Sindh, Pakistan
2
Department of Animal Sciences, University of Florida, Gainesville, FL, 32608, USA
3
Department of Maritime Sciences, Bahria University Karachi Campus, 75000, Karachi Sindh, Pakistan
4
Department of Zoology, Government College University Faisalabad, 38000, Faisalabad Punjab, Pakistan
5
Department of Zoology, University of the Punjab Lahore 05422, Pakistan
6
Department of Zoology, University of Sargodha, 40100, Punjab, Pakistan
7
Plant and Environmental Protection, National Agricultural Research Centre, Islamabad, 45500, Pakistan
8
Centre for Research on Fish Nutrition and Environmental Ecology of the Ministry of Agriculture, Shanghai Ocean
University, Shanghai 201306, China
9
Department of Zoology, Islamia College University, Peshawar 25120, Khyber Pakhtunkhwa, Pakistan
10
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 55760, Riyadh
11451, Saudi Arabia
Submission date: 2024-06-27
Final revision date: 2024-08-27
Acceptance date: 2024-10-07
Online publication date: 2025-02-25
Publication date: 2025-11-04
Corresponding author
Pol. J. Environ. Stud. 2025;34(6):7795-7803
KEYWORDS
TOPICS
ABSTRACT
This study evaluated the physiological effects of sublethal concentrations of Cd2+ and Pb2+, both
individually and in combination, on Channa marulius, an important species in local fisheries. A total
of 160 fish were divided into four groups (control, Cd2+-treated, Pb2+-treated, and Cd2+ + Pb2+ treated)
and exposed to 30% of the 96-hour LC50 values for CdCl2 (22.71 mg/L), PbCl2 (16.02 mg/L), and their
combination for 40 days. Results showed significantly (P < 0.05) higher Cd2+ and Pb2+ concentrations
in the tissues of exposed groups compared to controls, with the highest accumulation in the kidney,
followed by gills and intestines. Enzyme activities of catalase (CAT) and superoxide dismutase (SOD)
were significantly reduced, especially in the combined exposure group. Additionally, red blood cells, hemoglobin, and total protein levels decreased, while cortisol and glucose levels increased, indicating
stress. Acetylcholinesterase (AChE) activity in the gill and liver was also significantly inhibited in the
combined exposure group. These findings highlight the risks posed by combined heavy metal exposure
to fish physiology, underscoring potential threats to fish health and local fisheries.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
REFERENCES (50)
1.
NAZ S., FAZIO F., HABIB S.S., NAWAZ G., ATTAULLAH S., ULLAH M., HAYAT A., AHMED I. Incidence of heavy metals in the application of fertilizers to crops (wheat and rice), a fish (common carp) pond and a human health risk assessment. Sustainability, 14, 13441, 2022. https://doi.org/10.3390/su1420....
2.
RAHMAN Z., SINGH V.P. The relative impact of toxic heavy metals (THMs) (arsenic (As), cadmium (Cd), chromium (Cr) (VI), mercury (Hg), and lead (Pb)) on the total environment: an overview. Environmental Monitoring and Assessment, 191, 1, 2019. https://doi.org/10.1007/s10661... PMid:31177337 PMCid:PMC10998116.
3.
MADGETT A.S., YATES K., WEBSTER L., MCKENZIE C., MOFFAT C.F. The concentration and biomagnification of trace metals and metalloids across four trophic levels in a marine food web. Marine Pollution Bulletin, 173, 112929, 2021. https://doi.org/10.1016/j.marp... PMid:34534935.
4.
HABIB S.S., BATOOL A.I., REHMAN M.F.U., NAZ S. Assessment and bioaccumulation of heavy metals in fish feeds, water, and some tissues of Cyprinus carpio cultured in different environments (Biofloc Technology and Earthen Pond System). Biological Trace Element Research, 201, 3474, 2023. https://doi.org/10.1007/s12011... PMid:36201118.
5.
FAZIO F., HABIB S.S., NAZ S., HASHMI M.A.H., SAOCA C., ULLAH M. Cadmium sub-lethal concentration effect on growth, haematological and biochemical parameters of Mystus seenghala (Sykes, 1839). Biological Trace Element Research, 200, 2432, 2022. https://doi.org/10.1007/s12011... PMid:34373978.
6.
YANG G., TANG Y., LIU X., WANG L., QIN L., LI D., SHEN X., KONG C., ZHAI W., FODJO E.K., FAN C. Determination of Free Glycidol and Total Free Monochloropropanediol in Fish and Krill Oil with Simple Aqueous Derivatization and High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Foods, 13, 2340, 2024. https://doi.org/10.3390/foods1... PMid:39123532 PMCid:PMC11311869.
7.
TASLEEM S., MASUD S., HABIB S.S., NAZ S., FAZIO F., ASLAM M., ULLAH M., ATTAULLAH S. Investigation of the incidence of heavy metals contamination in commonly used fertilizers applied to vegetables, fish ponds, and human health risk assessments. Environmental Science and Pollution Research, 30, 100646, 2023. https://doi.org/10.1007/s11356... PMid:37639089.
8.
SHAHJAHAN M., TASLIMA K., RAHMAN M.S., ALEMRAN M., ALAM S.I., FAGGIO C. Effects of heavy metals on fish physiology-a review. Chemosphere, 300, 134519, 2022. https://doi.org/10.1016/j.chem... PMid:35398071.
9.
RIND K.H., HABIB S.S., UJAN J.A., FAZIO F., NAZ S., BATOOL A.I., ULLAH M., ATTAULLAH S., KHAYYAM K., KHAN K. The effects of different carbon sources on water quality, growth performance, hematology, immune, and antioxidant status in cultured Nile Tilapia with biofloc technology. Fishes, 8, 512, 2023. https://doi.org/10.3390/fishes....
10.
HABIB S.S., NAZ S., NAWAZ S., AMEER I., KHATOON A., REHMAN H.U., JAWAD S.M., ALI H. Comparative analysis of hematological parameters of some farmed and wild fish species. Pakistan Journal of Zoology, 54, 591, 2022. https://doi.org/10.17582/journ....
11.
HEYS K.A., SHORE R.F., PEREIRA M.G., JONES K.C., MARTIN F.L. Risk assessment of environmental mixture effects. RSC Advances, 6, 47844, 2016. https://doi.org/10.1039/C6RA05....
12.
GONG B., QIU H., ROMERO-FREIRE A., VAN GESTEL C.A., HE E. Incorporation of chemical and toxicological availability into metal mixture toxicity modeling: State of the art and future perspectives. Critical Reviews in Environmental Science and Technology, 52, 1730, 2022. https://doi.org/10.1080/106433....
13.
KARRI V., SCHUHMACHER M., KUMAR V. Heavy metals (Pb, Cd, As and MeHg) as risk factors for cognitive dysfunction: A general review of metal mixture mechanism in brain. Environmental Toxicology and Pharmacology, 48, 203, 2016. https://doi.org/10.1016/j.etap... PMid:27816841.
14.
BEISHON L.C., HOSFORD P., GURUNG D., BRASSARD P., MINHAS J.S., ROBINSON T.G., HAUNTON V., PANERAI R.B. The role of the autonomic nervous system in cerebral blood flow regulation in dementia: A review. Autonomic Neuroscience, 240, 102985, 2022. https://doi.org/10.1016/j.autn... PMid:35525173.
15.
ABUBAKAR M.U., ABUBAKAR D. Characterization of acetylcholinesterase from various sources: a mini review. Journal of Environmental Bioremediation and Toxicology, 4, 24, 2021. https://doi.org/10.54987/jebat....
16.
ROTUNDO R.L. Acetylcholinesterase at the neuromuscular junction. Neuroscience Letters, 735, 135157, 2020. https://doi.org/10.1016/j.neul... PMid:32540360.
17.
ORTEGA D.R., ESQUIVEL D.F.G., AYALA T.B., PINEDA B., MANZO S.G., QUINO J.M., MORA P.C., DE LA CRUZ V.P. Cognitive impairment induced by lead exposure during lifespan: Mechanisms of lead neurotoxicity. Toxics, 9, 23, 2021. https://doi.org/10.3390/toxics... PMid:33525464 PMCid:PMC7912619.
18.
LV Z., HU J., HUANG M., PAN G., XU G., YANG M. Molecular mechanisms of cadmium-induced cytotoxicity in human ovarian granulosa cells identified using integrated omics. Ecotoxicology and Environmental Safety, 272, 116026, 2024. https://doi.org/10.1016/j.ecoe... PMid:38290317.
19.
NAZIR S., KHAN N., FATIMA M., AZMAT H., NAVEED S., RAMZAN M.M., ASGHAR M., BANO S., KHIZER A., WAN A.H., DAVIES S.J. The influence of dietary protein concentration on digestive enzyme activities, growth, and body composition in juvenile bullseye snakehead (Channa marulius). PLoS One, 18, e0281274, 2023. https://doi.org/10.1371/journa... PMid:36787289 PMCid:PMC9928133.
20.
AHMED S.F., KUMAR P.S., KABIR M., ZUHARA F.T., MEHJABIN A., TASANNUM N., HOANG A.T., KABIR Z., MOFIJUR M. Threats, challenges and sustainable conservation strategies for freshwater biodiversity. Environmental Research, 214, 113808, 2022. https://doi.org/10.1016/j.envr... PMid:35798264.
21.
BATOOL M., ABDULLAH S., IJAZ M.U., KOUSAR S., FATIMA M., ILYAS R., MUGHAL K.T. Heavy metals (cadmium and lead) induced oxidative stress in Channa marulius and Wallago attu during acute toxicity experiments. Pakistan Journal of Zoology Supplementary Series, 13, 74, 2018.
22.
American Public Health Association (APHA), American Water Works Association (AWWA), Water Environment Federation (WEF). Standard methods for the examination of water and wastewater, 22nd edition, 2012.
23.
HABIB S.S., NAZ S., FAZIO F., CRAVANA C., ULLAH M., RIND K.H., ATTAULLAH S., FILICIOTTO F., KHAYYAM K. Assessment and bioaccumulation of heavy metals in water, fish (wild and farmed) and associated human health risk. Biological Trace Element Research, 202, 725, 2024. https://doi.org/10.1007/s12011....
24.
HABIB S.S., SAEED M.Q., MASUD S., KESBIÇ O.S., UJAN J.A., CRAVANA C., AL-REJAIE S.S., MOHANY M., FAZIO F. Dietary Effect of Withania somnifera Root Powder on Growth, Hematobiochemical Parameters, Immunity, and Disease Resistance to Aeromonas hydrophila in Cyprinus carpio. Aquaculture Nutrition, 2024, 7321287, 2024. https://doi.org/10.1155/2024/7... PMid:39555538 PMCid:PMC11458298.
25.
HABIB S.S., BATOOL A.I., REHMAN M.F.U., NAZ S. Comparative analysis of hemato‐biochemical profile, growth performance, and body composition of Common Carp cultured under different feed and habitats (biofloc technology and earthen pond systems). North American Journal of Aquaculture, 85, 277, 2023. https://doi.org/10.1002/naaq.1....
26.
AMOURI R.E., UJAN J.A., RAZA A., MUSHTAQ A., SAEED M.Q., MASUD S., HABIB S.S., MILOŠEVIĆ M., AL-REJAIE S.S., MOHANY M. Sublethal Concentrations of Cadmium and Lead: Effects on Hemato-Biochemical Parameters and Tissue Accumulation in Wallagu attu. Biological Trace Element Research, 1, 2024. https://doi.org/10.1007/s12011... PMid:38536638.
27.
TRINDER P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry, 6, 24, 1969. https://doi.org/10.1177/000456....
28.
HENRY R.J. Colorimetric determination of total protein: clinical chemistry. Harper and Row, New York, 1964.
29.
REITMAN S., FRANKEL S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28, 56, 1957. https://doi.org/10.1093/ajcp/2... PMid:13458125.
30.
PRETTO A., LORO V.L., MORSCH V.M., MORAES B.S., MENEZES C., CLASEN B., HOEHNE L., DRESSLER V. Acetylcholinesterase activity, lipid peroxidation, and bioaccumulation in silver catfish (Rhamdia quelen) exposed to cadmium. Archive of Environmental Contamination and Toxicology, 58, 1008, 2010. https://doi.org/10.1007/s00244... PMid:19946682.
31.
SABULLAH M.K., AHMAD S.A., SHUKOR M.Y., GANSAU A.J., SYED M.A., SULAIMAN M.R., SHAMAAN N.A. Heavy metal biomarker: Fish behavior, cellular alteration, enzymatic reaction and proteomics approaches. International Food Research Journal, 22, 435, 2015.
32.
TUNÇSOY M., ERDEM C. Accumulation of copper, zinc and cadmium in liver, gill and muscle tissues of Oreochromis niloticus exposed to these metals separately and in mixture. Fresenius Environmental Bulletin, 23, 1143, 2014.
33.
QU R., WANG X., WANG Z., WEI Z., WANG L. Metal accumulation and antioxidant defenses in the freshwater fish Carassius auratus in response to single and combined exposure to cadmium and hydroxylated multi-walled carbon nanotubes. Journal of Hazardous Materials, 275, 89, 2014. https://doi.org/10.1016/j.jhaz... PMid:24857893.
34.
AL-BALAWI H.F.A., AL-AKEL A.S., AL-MISNED F., SULIMAN E.A.M., AL-GHANIM K.A., MAHBOOB S., AHMAD Z. Effects of sub-lethal exposure of lead acetate on histopathology of gills, liver, kidney and muscle and its accumulation in these organs of Clarias gariepinus. Brazilian Archives of Biology and Technology, 56, 293, 2013. https://doi.org/10.1590/S1516-....
35.
MALARVIZHI A., SARAVANAN M., POOPAL R.K., HUR J.H., RAMESH M. Accumulation of cadmium and antioxidant and hormonal responses in the Indian Major Carp Cirrhinus mrigala during acute and sublethal exposure. Water Air Soil Pollution, 228, 1, 2017. https://doi.org/10.1007/s11270....
36.
ORR S.E., BRIDGES C.C. Chronic kidney disease and exposure to nephrotoxic metals. International Journal of Molecular Sciences, 18, 1039, 2017. https://doi.org/10.3390/ijms18... PMid:28498320 PMCid:PMC5454951.
37.
UNGUREANU E.L., MUSTATEA G. Toxicity of heavy metals. In: Environmental Impact and Remediation of Heavy Metals. IntechOpen, 2022.
38.
LIU X., DAI B., CHUAI Y., HU M., ZHANG H. Associations between vitamin D levels and periodontal attachment loss. Clinical Oral Investigations, 27, 4727, 2023. https://doi.org/10.1007/s00784... PMid:37291391.
39.
ATLI G., CANLI E.G., EROGLU A., DOGAN Z., CANLI M. Cadmium and lead alter the antioxidant and osmoregulation systems in the erythrocyte of fish (Oreochromis niloticus). Turkish Journal of Fisheries and Aquatic Sciences, 16, 361, 2022.
40.
RAJESHKUMAR S., LIU Y., MA J., DUAN H.Y., LI X. Effects of exposure to multiple heavy metals on biochemical and histopathological alterations in common carp, Cyprinus carpio L. Fish Shellfish and Immunology, 70, 461, 2017. https://doi.org/10.1016/j.fsi.... PMid:28826748.
41.
UNSAL V., DALKIRAN T., ÇIÇEK M., KÖLÜKÇÜ E. The role of natural antioxidants against reactive oxygen species produced by cadmium toxicity: a review. Advanced Pharmaceutical Bulletin, 10, 184, 2020. https://doi.org/10.34172/apb.2... PMid:32373487 PMCid:PMC7191230.
42.
CHUAI Y., DAI B., LIU X., HU M., WANG Y., ZHANG H. Association of vitamin K, fibre intake and progression of periodontal attachment loss in American adults. BMC Oral Health, 23, 303, 2023. https://doi.org/10.1186/s12903... PMid:37198606 PMCid:PMC10193670.
43.
FAZIO F. Fish hematology analysis as an important tool of aquaculture: a review. Aquaculture, 500, 237, 2019. https://doi.org/10.1016/j.aqua....
44.
HU R., FENG H. Lenticulostriate artery and lenticulostriate-artery neural complex: new concept for intracerebral hemorrhage. Current Pharmaceutical Design, 23, 2206, 2017. https://doi.org/10.2174/138161....
45.
SCHARF P., BROERING M.F., OLIVEIRA DA ROCHA G.H., FARSKY S.H.P. Cellular and molecular mechanisms of environmental pollutants on hematopoiesis. International Journal of Molecular Sciences, 21, 6996, 2020. https://doi.org/10.3390/ijms21... PMid:32977499 PMCid:PMC7583016.
46.
ZHEN W., WANG Z., WANG Q., SUN W., WANG R., ZHANG W., ZHANG Y., QIN W., LI B., WANG Q., HONG B. Cardiovascular disease therapeutics via engineered oral microbiota: Applications and perspective. iMeta, 3, e197, 2024. https://doi.org/10.1002/imt2.1... PMid:38898992 PMCid:PMC11183164.
47.
HUI M., JIA X., LI X., LAZCANO-SILVEIRA R., SHI M. Anti-Inflammatory and Antioxidant Effects of Liposoluble C60 at the Cellular, Molecular, and Whole-Animal Levels. Journal of Inflammation Research, 16, 83, 2023. https://doi.org/10.2147/JIR.S3... PMid:36643955 PMCid:PMC9833127.
48.
BUKHARAEVA E., KHUZAKHMETOVA V., DMITRIEVA S., TSENTSEVITSKY A. Adrenoceptors modulate cholinergic synaptic transmission at the neuromuscular junction. International Journal of Molecular Sciences, 22, 4611, 2021. https://doi.org/10.3390/ijms22... PMid:33924758 PMCid:PMC8124642.
49.
WANG Z., SUN W., HUA R., WANG Y., LI Y., ZHANG H. Promising dawn in tumor microenvironment therapy: engineering oral bacteria. International Journal of Oral Science, 16, 24, 2024. https://doi.org/10.1038/s41368... PMid:38472176 PMCid:PMC10933493.
50.
LIU M., DENG P., LI G., LIU H., ZUO J., CUI W., ZHANG H., CHEN X., YAO J., PENG X., PENG L. Neurotoxicity of combined exposure to the heavy metals (Pb and As) in zebrafish (Danio rerio). Toxics, 12, 282, 2024. https://doi.org/10.3390/toxics... PMid:38668505 PMCid:PMC11054020.
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| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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