ORIGINAL RESEARCH
Identification and Nitrogen Removal
Characteristics of a Denitrifying Strain
Yan Ji1,2, Haiyan Pei1,2, Wenrong Hu1,2
1School of Environmental Science and Engineering, Shandong University,
2Shandong Provincial Engineering Center on Environmental Science and Technology,
Jinan 250061, Shandong Province, China
2Shandong Provincial Engineering Center on Environmental Science and Technology,
Jinan 250061, Shandong Province, China
Pol. J. Environ. Stud. 2013;22(3):699-706
KEYWORDS
ABSTRACT
The nitrogen (N) removal characteristics of denitrifying bacterium LZ-14 were investigated in this study.
Extensive carbon sources can be used for LZ-14, with acetate as the most effective one. The strain LZ-14 grew
fast at a range of 20ºC to 35ºC, and exhibited high N-removal efficiency. Nitrogen removal mainly was performed
in the growth phase of 12-36 hr with an amount of nitrite accumulation that was reduced completely
in the following 24 hr. The strain LZ-14 was identified as Pseudomonas by PCR amplification and homology
analysis of 16S rDNA sequence. Strain LZ-14 was inoculated in simulative constructed wetlands (SCWs) to
enhance pollutant removal in pilot-scale, which showed a significant effect on CODCr and total nitrogen (TN)
removal.
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| eISSN: | 2083-5906 |
| ISSN: | 1230-1485 |
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