In this study, major ions, isotopes and rare earth elements (REEs) were tested in groundwater from the coal-bearing sandstone aquifer of the Wolonghu coal mine in northern Anhui Province, and the elemental geochemical behavior and controlling factors in groundwater were discussed. The results indicate that the overall sandstone water is weakly alkaline, and the hydrochemical type is SO₄-Na. The relationship between δD and δ¹⁸O suggests a hydraulic connection between the sandstone aquifer and the limestone aquifer. The composition characteristics of δ³⁴S_SO4 a nd δ¹⁸O_SO4 indicate that the high concentration of SO4^2- in groundwater mainly comes from the dissolution of sulfate minerals. The content of rare earth elements (ΣREEs) in groundwater is controlled by pH value and carbonate complexation reaction, and it shows a relative enrichment feature of heavy rare earth elements (HREEs) after standardization with Post-Archean Australian Shales. Ce shows a slight negative anomaly and is mainly influenced by the redox environment, while the Eu anomaly is attributed to the dissolution of silicate minerals. Compared to groundwater quality before the mine closure, the weakening of hydraulic conditions led to an increase in ΣREEs, Ca²⁺, Mg²⁺, SO4^2-, and TDS concentrations accompanied by a significant decrease in HCO₃^- concentration. In addition, the difference between light rare earth elements (LREEs) decreased, whereas the differentiation between LREEs and HREEs gradually increased. Our findings provide valuable insights into the evolution of groundwater quality in abandoned mines and its genesis mechanism. The results could guide the utilization of groundwater resources in abandoned mines.