ORIGINAL RESEARCH
How Nitric Oxide Down-Regulates
Cryptochrome 1 from Canola in Blue but Not
Red Light, and Reduces Hypocotyl Dwarfism
in Lead and Drought Stress
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Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Submission date: 2018-10-02
Final revision date: 2019-02-06
Acceptance date: 2019-02-17
Online publication date: 2019-10-24
Publication date: 2020-01-16
Corresponding author
Nahid Masoudian
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Pol. J. Environ. Stud. 2020;29(2):1161-1167
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ABSTRACT
Pollution of the environment and agricultural lands by lead and also drought stress is one of the
important factors in reducing the production and quality of crops, including canola (Brassica napus
L.). Expression of one key gene called cryptochrome 1 (CRY1) for reducing the hypocotyl dwarfism
was analyzed in canola seedlings (Hyola 401 cultivar). We have studied the CRY1 gene on canola
5-day-old seedlings after exposure to sodium nitroprusside as the nitric oxide (NO) donor (100 μM),
lead (100 μM), polyethylene glycol 6000 (PEG-6000) for imposed drought (5%) and their combination
(100 μM lead + PEG 5%). The blue and red lights were irradiated for 2, 4 and 8 hours (h). A protocol
for real-time quantitative PCR (RT-qPCR) analysis of this gene was developed using the β-actin gene as
an internal standard. The CRY1 gene significantly up-regulated in drought and simultaneous lead and
drought stresses in 8 h blue light irradiation. NO interaction considerably down-regulated the CRY1
gene in controlled seedlings and in all blue-light irradiations except for lead stress in 8 h. This light in
synchronous lead and drought stress led to significant hypocotyl dwarfism by CRY1, especially at 8 h.
Using NO interplay, hypocotyl dwarfism by CRY1 gene was reduced in all treatments except for red
light and lead stress in 4 and 8 h of blue light.