The Patagonian lakes have a glacial origin and some of these are associated with glaciers that generate specific optical properties, such as water colorations due to glacier sediments. These lakes also are oligotrophic with a low crustacean zooplankton species number. The aim of the present study was to analyze potential associations between optical properties and zooplankton communities in General Carrera Lake (46°S). The results revealed inverse associations in reflectance of bands 1, 5, and 7 of LANDSAT TM+ with calanoid copepodites, between band 7 with conductivity, and band 7 with B. michaelseni abundance. Also, we observed direct associations between bands 1-5 with total dissolved solids, meaning that this zooplankton assemblage is similar to north Patagonian oligotrophic lakes. These results would agree with a few reports for other similar Patagonian lakes of glacial origins as reported for Argentinean and Chilean Patagonia. Nevertheless, more studies are necessary for finding potential associations between limnological characters and optical properties.