This paper evaluates the ecotourism suitability of Henan province based on the ecotourism suitability evaluation system. And our research clarifies the spatial distribution of the ecotourism suitability in Henan province, China, explores the internal relations between landscape pattern and ecotourism suitability, and provides a scientific basis for the future sustainable development of ecotourism in Henan from an ecological perspective. The results show the following: 1) the ecotourism suitability is obvious differences between the eastern and western regions in Henan, with the indexes of ecotourism suitability in the western and southern regions being higher than those in other regions. The ecotourism suitability shows a trend of first increasing and then decreasing in Henan, with the highest value in 2007; 2) the Pearson correlation analysis shows that the key landscape pattern factors, including landscape patch area, shape, number, type, and spatial configuration, have an important impact on ecotourism suitability and show significant time differences.