The crop growing of sesame is of important significance due to its nutritional, medicinal, and biological value, which drives the need for improved production. Various environmental factors pose challenges to sesame yield, necessitating the development and promotion of cultivation technologies. One such method involves the application of plant growth-promoting rhizobacteria (PGPR) possessing enzymatic capabilities linked to plant growth promotion. In this study, rhizobacteria with enzymatic activity were isolated and screened, resulting in the identification of five promising strains (F-06, F-18, F-27, F-39, and F-45) from different investigative series. These strains, representing a variety of species, including Pseudomonas, Psychrobacter, and Bacillus, were assessed for their effects on field-grown sesame. The data revealed that bacterial inoculation led to notable improvements in various growth parameters of sesame compared to uninoculated controls. Specifically, inoculated plants exhibited increases of up to 26%, 12%, and 37% the plant, 1000-grain weight, and grain yield, respectively. Moreover, biotic yield, straw yield, and yield index were also significantly enhanced by 25%, 15%, and 17%, respectively, over inoculated controls. Additionally, there were substantial increases in oil content (47%) and protein content (62%) in response to inoculation with selected rhizobacteria compared to controls. These findings suggest the positive effect of multitrait microorganisms over PGPR and single-trait microbes when improving plant development and yield in sesame cultivation.