The necessity for the creation of safe, dependable, biocompatible, and efficient methods to make nanoparticles drives an increasing number of researchers to consider using biological systems as potential eco-friendly nanofactories. Fungi have an innate ability to reduce and oxidize metal ions into metal oxide nanoparticles, thus behaving as nanofactories. In the present study, Trichoderma harzianum was used to prepare ZnO nanoparticles (ZnO NPS). ZnO NPS showed a peak at 370 nm upon testing using UV spectrophotometer and percentages of zinc(72.0±0.2 %) and oxygen(28.0 ±0.3) upon analysis using Edx with an irregular shape, 16.5 nm size and characterized XRD pattern. Soymilk as well as the prepared ZnONPs were investigated either separately or in their mixture (1:1) for their antibacterial and anti-tumor effects. The mixture showed a promising antibacterial impact on Enterococcus faecalis (ATCC29212) and Escherichia coli (ATCC25922) which was confirmed using a transmission electron microscope. ZnONPs mixed with soymilk showed a promising anti-tumor action towards human colorectal adenocarcinoma cell line Caco-2 through boosting apoptotic rate, which was detected by flow cytometry. The current report highlights the possibility of mixing eco-friendly biosynthesized nanoparticles with natural products to enhance their biomedical impact with minimal toxicity as possible innovative pharmaceutical applications.