ORIGINAL RESEARCH
Enzymatic Activity of Urease and Degydrogenase
in Soil Fertilized With GWDA Compost
with or without a PRPSOL Addition
Ewa Krzywy-Gawrońska
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Department of Land Reclamation and Environmental Chemistry,
Western Pomeranian University of Technology in Szczecin,
Słowackiego 17, 71-434 Szczecin, Poland
Pol. J. Environ. Stud. 2012;21(4):949-955
KEYWORDS
ABSTRACT
In a two-year field experiment (2008-09), the effect of increasing doses of compost produced from municipal
sewage sludge with or without the addition of active substance PRP®SOL (PRP Technologies, France) on
urease and dehydrogenase activities during cultivation of winter wheat and spring rapeseed was studied. The
experimental design included a control treatment with standard mineral fertilization and three levels of organic
fertilization. The used compost doses were equivalent to 100, 200, and 300 kg N·ha-1. Organic fertilization
was carried out on 28 September 2007. In 2008 and 2009 the whole experimental area was fertilized with active
substance PRP SOL at a 150 kg·ha-1 dose and multicomponent fertilize Polifoska 6 at a 200 kg·ha-1 dose.
Due to the low nitrogen content in Polifoska 6 (6% N), urea top-dressing for winter wheat and spring rapeseed
was applied at a dose of 100 kg N·ha-1 in two times periods. Soil samples for chemical analyses were collected
from the arable layer (0-25 cm) under winter wheat four times: April, May, and July (soil sampling times
I to III), and after its harvest at the end of August 2008 (sampling time IV). In spring 2009, spring rapeseed
was sown in the same field, and soil samples for analyses were collected in the same times I to III (April, May,
and July), while soil sampling time IV following the rapeseed harvest fell at the beginning of August 2009. It
was found that fertilization with a triple dose of compost with a PRP SOL addition increased the soil pHKCl
value as compared to control soils. Higher organic carbon (OC), nitrogen (N), and phosphorus (P) contents were
found in the soils collected from experimental plots being fertilized with a double and a triple dose of municipal
sewage sludge compost with a PRP SOL addition. Significant increase in urease activity was found between
soil sampling times I and IV (specify the treatment here). The applied fertilization with single and a triple doses
on compost with or without PRP SOL increased the urease activity on average by 30.4 between soil sampling
times I and IV. The largest increase in dehydrogenase activity was observed between soil sampling times I and
III and in control objects. The applied fertilization with a triple dose of compost with or without PRP SOL
increased the dehydrogenase activity on average by 18.65% between soil sampling times I and III.
The applied organic fertilization together with active substance PRP SOL stimulated the enzymatic
activity of urease and dehydrogenase in all cases.