Textile floor coverings (TFC), an important element of equipment in homes and public buildings, are regarded as a settlement of house dust mites, and thus the cause of mite-induced allergy. It is believed that TFC should be removed from premises used by persons with a diagnosed allergy to house dust mites. On the other hand, TFC removal is associated with an increased air concentration of dust particles and dust-containing allergens. It is feasible to equip TFC with properties that can prevent the settlement and development of dust mites. The aim of this study was to evaluate TFC properties designed to protect against dust mite generation. The culture of Dermatophagoides farinae was used to test the efficacy of the direct anti-mite action. TFC acquired anti-dust mite properties through the addition of biologically active agents to the coated backing and mounting the same acaricides and anti-adhesive fluorocarbon dispersion on pile fibers. The results of our studies show that modified TFCs are characterized by anti-dust mite properties that protect against Dermatophagoides farinae.