Forest soil together with the mulch covering it, its chemical composition, oxygen conditions, pH, moisture, and food resources determine the specific species composition of edaphon. The aim of this study was to evaluate the effects of acidic runoff off the bark of a tree on the faunistic list of macro-pedofauna at different distances from the base of the trunk. The study was conducted in the central part of the Świętokrzyskie Mountains located in southeastern Poland. Stemflow of beech (Fagus silvatica L.) were collected using bands fitted around five trunks at a height of DBH. The composition of pedofauna was determined using the Morris frameworks mounted at three different distances from the trunks: 10 cm, 50 cm, and 100 cm. At the same points, the pH and the moisture content of the soil were measured. In the soil samples, we also determined heavy metals (Pb, Cd, Cr, Co, Cu, and Zn). The data allowed us to analyze the determinants of distribution of pedofauna, depending on the distance from the trunk of the surveyed trees. The data shows that the main factors affecting the change in the number of pedofauna are soil pH, heavy metals, and resistance of organisms against contamination. Least resistant to high pH and heavy metal content in the soil are Diplopoda, Collembola, and Araneae.