Chronic exposure to microplastics can affect DNA integrity and alter gene expression within living beings. The current study involved the dietary administration of varying doses of polyvinyl chloride microplastics (PVC-MPs) mixed with basal feed to five different groups of albino mice for thirty-five days and determining their potential toxicity in terms of DNA damage, micronucleus formation, oxidative stress, and gene expression. The results of the alkaline comet assay indicated a positive correlation between doses with increased DNA damage and fragmentation, with maximum damage observed in treatment 4 (0.8 mg/kg/BW). The damage index increased from 99 in T1 (0.1mg of MPs/kg/BW) to 219 in T4 (0.8mg of MPs/KG/BW) in liver cells, while for kidney cells, the value increased from 108 for T1 to 211 in T4. Likewise, a strong positive correlation was observed between the doses and Binuclei (BN) formation (n = 4; r = 0 .950), d oses a nd M icronuclei ( MN) f ormation (n = 4; r = 0.984), a nd b etween BN a nd M N (n = 4; r = 0.953) at t he 0.05 significance level. A n i ncrease i n m alondialdehyde ( MDA) levels indicated increased lipid peroxidation, with a proportional increase in MP doses administered to experimental groups. Gene expression for OGG1 and HPRT1 genes was determined, and a remarkable fold change was observed in relation to the increase in doses. The study’s findings conclude that an increase in microplastic exposure can cause significant damage to animal health.