The content of selected trace metals (Cd, Cr, Hg, Ph, Zn) and their distribution in superficial water, bottom sediments and in muscular tissue of fishes (roach, perch, bream) and mollusks (taken from the Zemborzyce Reservoir) were examined using atomic absorption spectrometry (AAS). A comparison of the trace elements content in investigated constituents of the Zemborzyce Reservoir was carried out. Superficial water contains micro-trace quantities of examined metals. Bottom sediments are characterized by a smaller content of Cd, Pb and Zn, in comparison to the previous data. Accumulation of Cd is very similar for all examined fishes. The highest level of Cr was found in roaches. Perches indicate best accumulation ability of Hg, Pb and Zn. Mollusks show excellent accumulation ability to all studied elements.