ORIGINAL RESEARCH
Detection of Phytopathogenic Bacteria
Damaging Weeping Birch (Betula Pendula)
by Molecular Identification Method
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1
L.N. Gumilyov Eurasian National University, Nur-Sultan, Kazakhstan
2
S. Seifullin Agrotechnical University, Nur-Sultan, Kazakhstan
Submission date: 2021-10-20
Final revision date: 2022-01-11
Acceptance date: 2022-01-28
Online publication date: 2022-08-30
Publication date: 2022-09-28
Corresponding author
Sardarbek Abiyev
L.N. Gumilyov Eurasian National University, Nur-Sultan, Kazakhstan
Pol. J. Environ. Stud. 2022;31(5):4549-4556
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ABSTRACT
The article discusses the morphological changes of birch trees due to bacterial dropsy growing in
the “Green Belt” artificial forest in the surrounding of the Nur-Sultan city. Samples of kernel were taken
from diseased tree trunks, and, afterward, isolated bacterial cultures were grown in a nutrient medium.
For molecular genetic studies, one strain (designated as DDdd-1), the most typical of morphological
characteristics, was selected, the colonies of which showed the greatest growth on nutrient media.
The nucleotide sequences 8F and 806R of 16SrRNA were determined on the basis of sequencing.
The resulting sequences were compared in GenBank from the NCBI database showing related
species and their similarities. Extraction of cores from infected birch trunks, isolation of pure strains
of the pathogen, molecular identification of ribosomal RNA 16S nucleotide chains were performed
using modern methods in the field of bacteriology. Then, the results obtained were compared with
relevant data from the International Genbank database. Molecular characteristics of isolated bacterial
DDdd-1 strain have been found to correspond to typical Dickeya dadantii by 96.72%. According to
the International Genbank, the DDdd-1 strain is defined as pathovar D. dadantii pv. betulae. The data
obtained demonstrate close relation of DDdd-1 strain Dickeya dadantii pv. betulae to some strains
of Dickeya chrysanthemi. The species identified in International Genbank as Erwinia chrysanthemi
with index numbers KJ541470.1 and AF373175.1 are assumed to be identical to D. dadantii pv. betulae.
Dickeya is a complex taxon that still requires a lot of research efforts on species composition, close
family bonds, the size and structure of host plants.