The content of DDT and its metabolites (p,p’-DDD and p,p’-DDE) was determined in the muscles, liver and brain of crucian carp (Carassius carassius L.), carp (Cyprinus carpio L.), pike (Essox lucius L.) and sunbleak (Leucaspius delineatus Haeckel) collected from breeding ponds in Knyszyn, Podlasie province. Two-stage column chromatography with Celit 545 and Florisil was used to extract DDT and its metabolites. Measurements were performed using a gas chromatograph, PYE Unicam 104 type, with an electron capture detector. Relatively low concentrations of polychlorine pesticides (including trace amounts) were found. The highest mean concentrations of DDTs were detected in the brain and liver (1.1650 and 0.5469 mg kg^-1 of tissue, respectively). In the muscles, the concentration was substantially lower ( 0.0530 mg kg^-1 of tissue).