In this study, heavy metal accumulation levels in females and males of the abdominal muscles of freshwater crayfish (Astacus leptodactylus Esch.,1823) were compared by using the atomic absorption spectrophotometry method in Kılıçkaya Reservoir. Accordingly, the average Cd 0.07±0.10, Cu 1.22±0.93, Cr 0.06±0.02, Pb 0.35±0.35, Zn 9.48±2.64 as μg/g levels in males of crayfish were determined to be higher than the average Cd 0.01±0.01, Cu 0.28±0.44, Cr 0.05±0.02, Pb 0.08±0.12, and Zn 3.69±2.29 as μg/g levels in females of crayfish. Data were compared statistically using an independent samples t test, the Mann Whitney U test, and cluster analysis. The most abundant heavy metal in muscle in both male and female crayfish was Zn. The differentiation of Cu and Zn accumulation according to gender was statistically significant (P<0.05). Cluster analysis performed separately in both female and male crayfish groups revealed two clusters close to each other with Cd, Cu, Cr, Pb, and distantly related to Zn. However, both male and female crayfish in the Kılıçkaya Reservoir are safe for consumption.