Objective: There are many differences among Chuanwu from different producing areas.Therefore, it is extremely urgent to make a comparative analysis of the quality of Chuanwu from different producing areas. It will provide a theoretical reference for the formulation of relevant quality standards for Chuanwu. Methodology: 48 batches of Chuanwu samples were collected from Sichuan Province, Shaanxi Province, and Yunnan Province, and the environmental investigation was carried out in the main production areas. Results: There are significant differences in the quality of Chuanwu among the four producing areas. The content and proportion of neoline and zongorine are different, which can effectively identify Chuanwu from different producing areas. Conclusion: The appearance characteristics and alkaloid content of Chuanwu from different producing areas are closely related to climatic conditions and planting management. In this study, the quality identification index of Chuanwu from each producing area was preliminarily established.