This article contains measurements proving the benefits of biobutanol in methyl ester fuels made from animal fats. Generally, methyl esters from animal fats have much worse fuel parameters than rape seed methyl esters, for example. The tested biobutanol in this article has improved properties of produced methyl ester. Butanol is more suitable than bioethanol for diesel engines. Fuel properties of density, kinematic viscosity, behaviour of fuel under low temperatures, and flash point and cetane number for different butanol content in fuel blend were tested. It was shown that butanol improves the quality of methyl ester fuels from animal fats, especially its behaviour under low temperatures. Adding biobutanol to blends creates from animal fat methyl ester a more attractive fuel. Furthermore, using animal fat waste for methyl ester production and waste lignocellulose for biobutanol production are considered to be advantageous by the EU as being more sustainable for isolation of waste transfers between regional, natural, and industrial circles of the circular economy.