ORIGINAL RESEARCH
Assessment of Changes in Microbial Communities
in Different Operational Units from a Wastewater
Treatment Plant
Nilgün Poyraz, Mehmet Burcin Mutlu
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Department of Biology, Faculty of Science, Anadolu University
TR 26470 Eskisehir, Turkey
Submission date: 2016-11-22
Final revision date: 2017-02-03
Acceptance date: 2017-02-08
Online publication date: 2017-06-19
Publication date: 2017-07-25
Pol. J. Environ. Stud. 2017;26(4):1615-1625
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ABSTRACT
Microbial communities in wastewater play an important role in treatment, and especially microbial
community composition and changes have great influence on biological treatment. In these cases, monitoring
and evaluating the changes with practical methods becomes important. Ecoplates may be an effective and
quick method for assessing microbial activity. Therefore, we observed, with Ecoplates, the microbial
community function of different operational units from wastewater treatment plants.
Wastewater and sludge samples used in the study were taken from different units of a sewage treatment
plant located in the Eskisehir in two different time intervals. Changes in wastewater samples’ metabolic
diversity and microbial activity were evaluated. High similarity rates were obtained from samples, as
indicated by the diversity indices and average well color development (AWCD) values. A return activated
sludge pump and anaerobic digestion unit were highly active in the community. Low substrate usage
rates were observed in the secondary classifier unit and the lowest used substrate was 2-hydroxybutyric
acid from the carboxylic acid group. The most actively used substrates were from the carbohydrate group
substrates. These results refer to the degradation capacity. In addition, results showed that sampling intervals
and operational conditions caused changes in the functional diversity of communities. This study allowed
us to gather general information about microbial community structures in different operational units of
wastewater treatment systems. The results obtained from the study showed that this method is useful for
monitoring the treatment process, bacterial changes, and operational parameters.