ORIGINAL RESEARCH
Antifungal, Antibacterial, and Antioxidant
Activities of Camel Whey Protein Hydrolysates
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Department of Biology, College of Science Princess Nourah bint Abdulrahman University,
P.O. Box 84428, Riyadh 11671, Saudi Arabia
Submission date: 2023-08-13
Final revision date: 2023-11-13
Acceptance date: 2023-12-31
Online publication date: 2024-05-20
Publication date: 2024-06-07
Corresponding author
Albandary Nasser Alsaloom
Assistant Professor of Microbiology, Department of Biology, College of Science
Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia, Saudi Arabia
Pol. J. Environ. Stud. 2024;33(4):4517-4524
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ABSTRACT
In the current study, camel whey protein (CWP) was hydrolyzed with alcalase for 12 h
at an enzyme/substrate ratio of 1/200 (w/w). The antioxidant activity, antibacterial activity,
and antifungal activity of the hydrolysates at different times (2, 4, 6, 8, 10, and 12 h) were evaluated.
The highest DH is 47%, which was obtained after 12 h in comparison with those obtained
after 2, 4, 6, 8, and 10 h (20, 26, 32, 39, and 47%, respectively). After 8 h, proteolysis presented
the highest antioxidant activity against DPPH free radical (70% at a concentration of 200 μg/mL).
The bacteria S. typhimurium and E. coli were most affected, with MIC values of 78 and 625 μg mL-1,
respectively. The fungal growth of B. cinerea was reduced by 44%, 56%, and 78%, respectively,
when CWPH was administered at 250, 500, and 1000 μg mL-1. It can be concluded that camel whey
protein hydrolysates have antibacterial, antioxidant, and antifungal activity against pathogenic bacteria
and fungi.