Contents of cadmium, cobalt, chromium, nickel, and lead were determined in tubers of Jerusalem artichoke (Helianthus tuberosus L.) obtained from a field experiment carried out in 2003-05. The experimental factors consisted of two cultivars of Jerusalem artichoke (Albik and Rubik), plus different nitrogen nutrition levels on a background of constant phosphorus-potassium fertilization and of manure (FYM). The following experimental objects were formed: 1) control N₀P₀K₀, and the following mineral objects: 2) N₀P₄₄K₁₂₅, 3) N₅₀P₄₄K₁₂₅, 4) N₁₀₀P₄₄K₁₂₅, 5) N₁₅₀P₄₄K₁₂₅, and 6) N₂₀₀P₄₄K₁₂₅. Mineral fertilizers were not applied to the control object, but only manure (natural fertilizer) at the rate of 30 t·ha^-1. The Rubik cv. accumulated more heavy metals than Albik cv.; the latter was characterized by less variability of heavy metals content. When applying constant phosphorus-potassium fertilization, the lowest cadmium content was recorded in combinations including 150 kg N·ha^-1, cobalt – 100 kg N·ha^-1, chromium (only phosphorus-potassium fertilization was used), as well as nickel and lead – in control object with no mineral nutrition. In both varieties of Jerusalem artichoke different amounts of heavy metals were collected due to the different mineral fertilization.