Reliable information that allows us to estimate the state of the environment and to forecast changes in the ecosystem are constantly required. Increasing environmental consciousness and dynamic development of analytical techniques are the main reasons for determining the wide range of pollutants occurring at very low concentrations in complex matrix samples. The presence and concentration of many of those pollutants in the environment are not yet subjected to legal regulations, so development of new methodologies to ensure good quality of results and their control are essential. This paper presents the target and development directions currently observed in environmental monitoring and biomonitoring. A lot of attention has been paid to a wide range of compounds that are endocrine disruptors. The overview of analytical approaches and procedures used to detect and to determine biologically active compounds can be found in this paper as well.