This research takes Wuhan as a case study with a focus on forest ecological network analysis based on an integrated approach. This approach applies the improved least-cost path model and scenario analysis to identify and compare networks with the cost surface setting, including ecological service value and biodiversity index. We proposed cost-based δ index and patch connection index (PCI) to evaluate the whole network structure and ecological source connection, as these two indexes comprehensively consider spatial structure and species migration cost. This study proves that the improved line kernel density method based on species migration cost is an effective tool to identify conservation priority areas and break points. Based on the proposed method, we identify and evaluate four types of urban forest ecological networks from different cost setting. The forest ecological network is uneven in spatial distribution. Overall ecological network structure has poor connection. We illustrate conservation priority areas and divide these priority areas into three categories of ecological security.