Our study evaluates the protective effect of the chelating agent N-(2-hydroxy ethyl ethylene diamine triacetic acid) [HEDTA] with and without propolis against Al(NO₃)₃- induced toxicity in liver, kidney, and brain. Toxicity was induced by a single administration of Al(NO₃)₃ at a dose of 6.5 mg/kg, intraperitoneally. HEDTA, propolis, and a combination of HEDTA+propolis were administered for 3 days after 24 h of Al exposure. Significant enhancements in AST, ALT, ALP, cholesterol, triglycerides, urea, uric acid, protein, and blood sugar were found, whereas albumin was decreased after Al exposure. The fall in GSH contents and increase in LPO were significant in hepatic, renal, and neuronal tissues. Al(NO₃)₃ caused significant inhibition in the activity of adenosine triphosphatase, superoxide dismutase, and catalase. It inhibited AChE activity in forebrain, midbrain, and hindbrain. Individual treatment of HEDTA and propolis restored biochemical parameters moderately toward control, but combined treatment of HEDTA+propolis showed better results than monotherapy. Combined treatment of HEDTA+propolis reduced oxidative stress and regained histological features and metabolic enzymes of liver, kidney, and brain toward control.